Abstract:
Brevetoxin discrimination and quantification is costly and time consuming, often preventing
analyses from being performed at sufficient spatial and temporal resolution. Development of an insitu
brevetoxin analyzer could allow for remote monitoring capabilities and keep the required human
interaction and costs to a minimum. In this work, a method for brevetoxin analysis suitable for
employment by a new in-situ HPLC is developed using individual HPLC and UV-VIS spectrometer
components and brevetoxin standards. Multiple light sources, spectrophotometer, liquid-core
waveguide, HPLC column, and eluent configurations were tested, and scripts for data-signal
processing were created. Initial results are promising and demonstrate proof-of-concept for a fully
functional in-situ brevetoxin detector.
